11.09.2022
14:00 – Registration
16:00 Afternoon session (chair: Ariane Briegel)
16:00 Ariane Briegel / Friedrich Förster
16:15 Peter Peters (Maastricht University)
tba
16:40 Florian Fäßler (Institute of Science and Technology Austria)
Arp2/3 Complex Isoform Diversity Governs Lamellipodial Actin Network Architecture
17:00 Elizabeth Wright (University of Wisconsin Madison)
tba
17:25 Ino AGRAFIOTI (European Research Council)
Applying for a grant of the European Research Council
18:00 Dinner
19:30 Keynote Lecture (chair: Friedrich Förster)
Wolfgang Baumeister (Max Planck Institute of Biochemistry)
Cryo-electron tomography: The Power of Seeing the Whole Picture
21:00 Bar
12.09.2022
08:30 Morning session I (chair: Achilleas Frangakis)
08:30 Gaia Pigino (Human Tecnopol, Milan)
tba
08:55 Kelly Lee (University of Washington)
Visualization of viral protein-mediated membrane fusion cryo-electron tomography
09:15 Christopher Ackerson (Colorado State University)
A Clonable Selenium Nanoparticle (cSeNP) In Cellular Tomography
09:35 Stefan Raunser (Max Planck Institute for Molecular Physiology)
tba
10:00 Break
10:30 Morning session II (chair: Peijun Zhang)
10:30 Alex DeMarco (Monash University)
Automated cryo-liftout
10:55 Oleg Sitsel (Max Planck Institute for Molecular Physiology)
TBA
11:15 Gregor Weiss (ETH Zürich)
Dissecting the lifecycle of uropathogens by directly analyzing patient samples with cryo-ET
11:35 Julia Mahamid (EMBL Heidelberg)
Enabling discovery by in-cell structural biology
12:30 Lunch
14:00 Afternoon session I
14:00 Martin Pilhofer (ETH Zürich)
tba
14:25 Moritz Wachsmuth-Melm (Heidelberg University Hospital)
Characterization of vRNP clustering in influenza A virus-infected cells using in-situ cryo-electron tomography
14:45 Benedikt Wimmer (University of Zurich)
A link between genotype and structural phenotype in core microbiome members revealed by cryo-ET
15:05 Peijun Zhang (Oxford University / Diamond light Centre)
Visualizing macromolecular structures in situ by cryo-electron tomography and subtomogram averaging
15:30 Break
16:00 Afternoon session II
16:00 David Mastronarde (University of Colorado Boulder)
Optimizing Tilt Series Alignment Parameters by Cross-validation
16:25 Sagar Khavnekar (Max Planck Institute of Biochemistry)
PlasmaFIB for high throughput in situ cryo-ET
16:45 Emily A. Machala (Center for Structural Systems Biology, Hamburg)
Signpost origami tags (SPOTs) for identifying Herpes Simplex Virus 1 membrane proteins in native environments
17:05 Achilleas Frangakis (Goethe University Frankfurt)
Sticky spots: Adhesion mechanisms for cell-cell communication and infection
18:00 Dinner
19:30 Poster session (Odd numbers)
21:00 Bowling Evening
13.09.2022
08:30 Morning session I (chair: Arjen Jakobi)
08:30 Ohad Medalia (University Zürich)
tba
08:55 Hugo van den Hoek (Biozentum, University of Basel)
In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains
09:15 Camilla Ventura Santos (MRC Laboratory of Molecular Biology)
CryoET of microtubules and their interactors inside cells
09:35 Wanda Kukulski (University Bern)
Architecture of membrane contact sites between ER and mitochondria
10:00 Break
10:30 Morning session II (chair: Sharon Wolf)
10:30 Jürgen Plitzko (Max Planck Institute of Biochemistry)
tba
10:55 Josie Ferreira (CSSB, Hamburg)
Luminal helices and microtubule giants revealed in the malaria parasite
11:15 Daniela Nicastro (University of Texas Southwestern)
The molecular organization and function of cytoskeletal assemblies
11:40 Dorit Hanein (Institut Pasteur)
tba
12:30 Lunch
14:00 Meet the sponsors
16:00 Afternoon session II (chair: Julia Mahamid)
16:00 Martin Beck (Max Planck Institute of Biophysics)
tba
16:25 Jun Liu (Yale)
Visualizing a bidirectional rotary motor in action
16:50 Bettina Zens (Institute of Science and Technology Austria)
Characterizing the architecture of native extracellular matrix by cryo-electron tomography
17:10 Wen Yang (Thermo Fisher Scientific)
Towards high-resolution and high-throughput in-situ structural biology
18:00 Dinner
19:30 Poster session (Even numbers)
14.09.2022
08:30 Morning session I (chair: Wanda Kukulski)
08:30 Grant Jensen (Caltech / Brigham Young University)
Highlights from recent cryo-ET at Caltech: two new high-res sub-tomogram averages and a couple technical advances
08:55 Wenfei Song (Utrecht University)
Rubrerythrin encapsulates encapsulins in archaea
09:15 Ricardo Righetto (Biozentum, University of Basel)
HDCR filaments and higher-order assemblies revealed by in situ cryo-ET
09:35 Janet Iwasa (University of Utah)
Animating Molecular Machines
10:00 Break
10:30 Morning session II
10:30 Sharon Wolf (Weizmann Institute of Science)
New developments in cryoSTEM tomography
10:55 Herman Fung (EMBL)
Genetically encoded multimeric labels for protein localisation in cellular cryo-electron tomography
11:15 Tzviya Zeev-Ben-Mordehai (Utrecht University)
Combining cellular tomography with in-cell cross-linking mass spectrometry to identify supramolecular arrays at the mitochondria-cytoskeleton interface
11:35 Bram Koster (Leiden University Medical Centre)
Electron tomography workflows in flux
12:30 Lunch